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KMID : 1141020180180010075
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Journal of Applied Oriental Medicine
2018 Volume.18 No. 1 p.75 ~ p.83
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Effect of Artemisia capillaris Thunberg on Anti-oxidative and Inhibition of Adipogenesis in 3T3-L1 Cells
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Lee Jin-A
Kim Min-Ju
Roh Seong-Soo
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Abstract
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Objectives : Obesity is the metabolic diseases caused by an imbalance between food intake and energy consumption. This is a factor that increases the incidence of various diseases such as hyperlipidemia, hypertension and diabetes. The purpose of this study was to investigate the antioxidant and anti-obesity effects of Artemisia capillaris Thunberg.
Methods : The anti-oxidant activities of Artemisia capillaris Thunberg were measured by DPPH and ABTS radical scavenging assay, total poly phenol and flavonoid. In this study, anti-obesity effects were confirmed by oil red O staining after MTT assay. The extracts were treated with 0.05, 0.1, 0.15, 0.2, 0.5, 1, 1.5, and 2 ㎎/㎖ to examine the effect of toxicity on 3T3-L1 cells. oil red O staining was performed at concentrations (0.1, 0.15 and 0.2 ㎎/㎖) that did not exhibit toxicity.
Results : The polyphenol and flavonoid contents of Artemisia capillaris Thunberg were 49.49±0.36 ㎎/g and 62.41±0.76 ㎎/g. The antioxidant effect on DPPH and ABTS radical scavenging increased in dose-dependent respectively. oil red O results triglyceride accumulation was decreased in a concentration-dependent manner.
Conclusion : Artemisia capillaris Thunberg reduced triglyceride accumulation in differentiated 3T3-L1 cells, compared with positive control cells.
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KEYWORD
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Artemisia capillaris Thunberg, 3T3-L1, anti-oxidant, anti-obesity
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